Applying Gene Silencing Technology to Contraception

Tomato and Tobacco Phytoene Desaturase Gene Silencing by Virus-Induced Gene Silencing (VIGS) Technique

Background and Aims: Virus-Induced Gene Silencing (VIGS) is a virus vector technology that exploits antiviral defense mechanism. By infecting plants with recombinant viruses containing host genes inserted in the viral genome, VIGS achieves the RNA silencing process. The purpose of this study was to investigate the possibility of tomato (Lycopersicon esculentum Mill.) and tobacco (Nicotiana be...

Sugarcane Mosaic Virus-Based Gene Silencing in Nicotiana benthamiana

Background:Potyvirus-based virus-induced gene silencing (VIGS) is used for knocking down the expression of a target gene in numerous plant species. Sugarcane mosaic virus (SCMV) is a monopartite, positive single strand RNA virus. Objectives:pBINTRA6 vector was modified by inserting a gene segment of SCMV in place of Tobacco rattle virus (TRV) genome part 1 (TRV1 or RNA1)...

The Effect of Chalcone Isomerase (Chi) Gene Silencing on Flavonoids Content in Petunia hybrida using RNAi Technology

have been bred with altered flower color using genetic engineering approaches. One of the most effective applications is the reduction of flower pigments by suppression of involved enzymes in their biosynthesis pathways. RNA interference (RNAi) has provided an effective tool for the knock down of genes involved in the production of flower pigments. In this study, a chi-RNAi construct was design...

Gene Silencing I A virus defence pathway and a technology

Applying Distributed Object Technology to Network Planning

The aim of this paper is to describe a generic interface through which a planning tool or design aid can be connected to a telecommunications network. In today’s complex and ever changing networks, network operators need to plan their networks very accurately and rapidly. Sacrificing accuracy generally has severe impacts on the scalability and maintainability of the network at a later stage. A ...